FIG. 4.

Northern hybridization analysis of the new acetyltransferase gene transcript in the wild-type NRRL 3299 strain, the Tri10-disrupted transformant (ΔTri10), the Tri10-overproducing transformant (↑Tri10), and the Tri6-disrupted transformant (ΔTri6). RNA blots, prepared with total RNA isolated for each strain from mycelia collected from 23-h YEPD-5G liquid shake cultures, were hybridized with an α-³²P-labeled gene-specific probe. rRNA bands in the corresponding ethidium bromide-stained gel prior to blotting are shown.