TABLE 1.
Construction information for plasmids carrying Tri1 flanking regions
| Plasmid | Cloning vector | Restriction enzymea | Size of PCR insert (kb) | Primers (sequences [5′-3′]) |
|---|---|---|---|---|
| pIKE1 | pCR-Script | KpnI | 2.1 | A-78 (GGTAGTGATCAGGGTTCGTC), A-65 (CATATCGAACCGGAAGTCCTCC) |
| pIKE5 | pCR-Script | XbaI | 2.5 | A-79 (GGTAGTGATGCCGAATAGAGG), A-81 (CTATGGTAGTGTGACCCTCTTG) |
| pAWP72 | pT7Blue-2 | PstI | 1.5 | A-65 (CATATCGAACCGGAAGTCCTCC), A-66 (GGCGATGTTCAAATCTGCCTGG) |
a
Restriction enzyme used to prepare genomic DNA samples for chromosome crawling via inverse PCR.