Table 3.

Dye solution retention by rat kidneys

Treatment	μg/g
Controls	163 ± 8.3
+TNFα	456 ± 41.8*
+EDTA	178 ± 7.4
+TNFα+EDTA	298 ± 14.5*§

The table reports the modification of vascular permeability; following in vivo treatment with EDTA and TNFα (see Methods section). The rat right kidney was in vivo perfused with trypan blue solution; washed with saline; removed; homogenized and centrifuged. The supernatants were run on a spectrophotometer at 540 nm wavelength. The data was then expressed as μg dye retained per weight (g) of fresh kidney. Maximum dye retention (dye perfusion without washing) yielded a value of 619 ± 24.7.

*p < 0.05 vs. controls; ^§p < 0.05 vs. TNFα