TABLE 4.
Inhibitor (200 μM) | % Inhibition of activity |
|
---|---|---|
Cytochrome P450 2B4 (100 μg/ml) | Rhodococcus sp. strain DN22 (5 mg [wet wt]/ml)b | |
Control (no inhibitor) | 0 | 0 |
Ellipticine | 75 ± 4d | 76 ± 7 |
Metyrapone | 68 ± 8 | 60 ± 6 |
Phenylhydrazine | 70 ± 7 | 77 ± 5 |
1-Aminobenzotriazole | 55 ± 7 | 43 ± 7 |
Carbon monoxidec | 82 ± 6 | 48 ± 5 |
The RDX transformation activity without inhibitor was considered 0% inhibition; the 100% activities for cytochrome P450 2B4 and Rhodococcus sp. strain DN22 were 9.0 nmol min−1 mg of protein−1 and 0.12 nmol min−1 mg of biomass−1, respectively.
A washed and centrifuged cell pellet was used as wet biomass.
Carbon monoxide was bubbled through the aqueous phase and headspace for 60 s in sealed vials.
The values are means ± standard deviations (n = 3).