TABLE 3.

Relative RNA levels of genes in DNA microarray comparisons of global gene expression in E. coli MG1655 cells grown for ∼20 generations on various media^a

Gene		Operon organization	Function	Avg relative mRNA levels for acetate adaptation/propionate adaptation
Designation	Name
b0001	thrL	↓	thr operon leader peptide	0.93
b0002	thrA	↓	Aspartokinase I, homoserine dehydrogenase I	0.53*
b0003	thrB	↓	Homoserine kinase	0.51*
b0004	thrC	↓	Threonine synthase	0.60*
b0929	ompF	↑	Outer membrane protein 1a (la;b;F)	2.18*
b2702	srlA1	↓	PTS, glucitol/sorbitol-specific IIC component	0.32*
b2703	srlA2	↓	PTS, glucitol/sorbitol-specific IIB component	0.37*
b2704	srlB	↓	PTS, glucitol/sorbitol-specific enzyme IIA component	0.38*
b2705	srlD	↓	Glucitol (sorbitol)-6-phosphate dehydrogenase	0.41*
b2706	gutM	↓	Glucitol operon activator	0.55
b2707	srlR	↓	Regulator for gut (srl), glucitol operon	0.65*
b2708	gutQ	↓	ORF, hypothetical protein	0.93
b3509	hdeB	↑	ORF, hypothetical protein	1.64*
b3510	hdeA	↑	ORF, hypothetical protein	2.10*
b3774	ilvC	↓	Ketol-acid reductoisomerase	0.19*

^aMedia tested were LB-20 mM sodium acetate and LB-20 mM sodium propionate. Asterisks indicate significant RNA level differences (P value, <0.05; Student's t test) that exceeded 2-fold or were below 0.5-fold. For operons, RNA levels for all genes are given. Three independent cultivations and DNA microarray experiments were performed.