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QRT-PCR analysis of PN-1, PAI-1 and uPA mRNA expression in MCF-7 and MDA-MB-231 breast cancer cell lines. PN-1, PAI-1 and uPA message levels were quantitated in MCF-7 and MDA-MB-231 cells by QRT-PCR. Expression levels for PN-1, PAI-1 and uPA were normalized to values obtained for TATA binding protein. CT values for each gene obtained from MCF-7 cells were averaged and assigned a value of one to assess relative fold increase in expression in MDA-MB-231 cells.
