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. 2000 Oct;11(10):3525–3537. doi: 10.1091/mbc.11.10.3525

Figure 6.

Figure 6

Northern analysis of sporulation-specific gene expression and dityrosine fluorescence in mps1 mutant strains. (A) MPS1 (YUMY125 × YUMY126) and mps1-1237 (YUMY119 × YUMY120) strains were synchronously sporulated, and aliquots were removed for RNA isolation. Filters were probed for expression of three midlate sporulation-specific genes, SMK1, SWM1, and DIT1, the late spore wall–specific gene SPS100, and the ACT1 gene as a loading control. Expression of SMK1 and SWM1 (our unpublished results) is unaffected in the mps1-1237 strain compared with wild type. The expression of the SPS100 gene is severely diminished in the mutant strains, whereas DIT1 expression is enhanced above wild-type levels. (B) Dityrosine fluorescence is detected in both mps1 mutant strains producing two- to four-spored asci. Patches of MPS1 (YUMY3E3), mps1-1237 (YUMY3I1), and mps1-412 (YUMY 1980) were sporulated at the restrictive temperature and then treated to activate dityrosine fluorescence. The presence of fluorescent material in wild-type and mps1 mutant strains indicates that insoluble fluorescent dityrosine is produced efficiently in both strain types.