FIG. 3.

Soluble HAV antigens from cells harboring the parental pBTac2 (square) or the recombinant pTHAVF (circle) plasmids at 4 h postinfection (A). Fractions corresponding to the 14S peak were pooled and submitted to a second sucrose gradient centrifugation (B). HAV detection was performed as described for Fig. 2.