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. 2006 Jul 13;116(8):2183–2192. doi: 10.1172/JCI24767

Figure 2. Regulation of aP2 expression in HBEs is enhanced by IL-4 and IL-13.

Figure 2

(A) Time course of aP2 induction in HBEs after 1, 3, 6, 12, 18, 24, 48, and 72 hours or of mal1 induction after 3, 6, 18, 24, and 48 hours, using real-time PCR activation after treatment with IL-4 or IL-13. Data represent mean ± SEM of 3 experiments. The data for the one-hour time point represent the mean of duplicate experiments. (B) BEAS-2B and A549 cells were treated with IL-4 or IL-13 for 18 hours and aP2 expression assayed by real-time PCR. (C) HBEs were stimulated with BSA (Ctrl), IL-4, or IL-13 for 24 hours and stained with anti-aP2 or isotype control antibodies with immunofluorescence. Images are representative of 3 experiments. Original magnification, ×400.