Figure 9. A_2B AR transcript and adenosine levels in the lungs.

(A) RNA was extracted from whole lungs of postnatal day 38 mice for analysis of A_2BAR expression using quantitative RT-PCR. Data are mean transcript levels ± SEM. *P ≤ 0.05 versus vehicle-treated ADA⁺; ^#P ≤ 0.05 versus vehicle-treated ADA^–/–. n = 4 (ADA⁺), 8 (ADA^–/–). (B) Adenine nucleotides were extracted from whole lungs of postnatal day 38 mice, and adenosine levels were quantified by reverse-phase HPLC. Data are mean fold increase ± SEM. *P ≤ 0.05 versus ADA⁺. n = 4 per group.