Table 1.

Cell proliferation in vitro, Day 7^*

	Without soluble enzyme	With soluble enzyme
Untreated matrix	1.0	1.6
Purified collagenase	2.3	4.5
Purified collagenase + HbEGF	2.9	4.9
HbEGF alone	1.4	2.6
Crude collagenase	1.2	2.3
Clostripain	1.1	2.0
Papain-Urea/chlorophyllin	0.5

*HbEGF indicates heparin-binding epidermal growth factor. Results shown are the comparative effects of matrix pretreatment and soluble factors on proliferation. Soluble enzymes are the same as those used for matrix pretreatment; cells plated on untreated matrix received soluble Clostridial collagenase. All soluble enzymes were added at 16 U/mL in the culture media. HbEGF was applied both in the presence and absence of soluble enzyme in the indicated samples. The number of cells in the control condition was set equal to one, and all other results are shown in relation to this control. For each condition, the average of at least 3 separate experiments is shown. All standard deviations are less than 0.05.