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. 2005 Apr;7(4):348–355. doi: 10.1593/neo.04544

Figure 4.

Figure 4

Interaction of WDRPUH with HSP70, CCT1-δ, CCT1-α, or BRCA2. HEK293 cells were transiently transfected with p3xFLAG-WDRPUH or p3xFLAG empty vector (Mock). Cell lysates were immunoprecipitated with anti-FLAG M2 antibody (mouse) conjugated with agarose beads or specific antibodies to interacting protein. (A and B) Coimmunoprecipitation of FLAG-WDRPUH and endogenous HSP70. Whole cell extracts from the cells exogenously expressing FLAG-WDRPUH were used as positive controls. (C) Coimmunoprecipitation of FLAG-WDRPUH and endogenous CCT1-δ. (D) Coimmunoprecipitation of FLAG-WDRPUH and endogenous CCT1-α. (E and F) Coimmunoprecipitation of FLAG-WDRPUH and endogenous BRCA2.