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Wild-type p53-dependent repression of SAK expression. H1299-V138 (A) or H1299/Neo (B) cells were grown either at 39°C (mutant p53 status) or 32°C (wild-type p53 status) for 6, 16, and 24 hours in the presence of DMSO or etoposide (25 µM) as indicated. Cells were harvested and RNA were prepared for Northern analysis (15 µg of total RNA) using probes against SAK. GAPDH was used as a loading control.
