Table 4.
Comparison of Microarray and Real-Time PCR Data: mRNA Expression in Seminoma Compared to Normal Testis Regarding Data from the Literature.
| Gene | Microarray | RT-PCR | Literature | |
| NT (n = 3), pT1 (n = 22), pT2 (n = 14), pT3 (n = 4) | NT (n = 9), pT1 (n = 8), pT2 (n = 14) | Testis [11] | CIS [14] | |
| IGF-1 | Constant | Constant | mRNA not detected | IHC positive |
| IGF-R1 | Constant | Constant | mRNA in germinal epithelium | |
| IGF-2 | 2x no expression, 1x increase in pT1 and pT3 | Decreased | mRNA in vessels, peritubular tissue, and connective tissue | IHC negative |
| IGF-R2 | Constant | Constant | mRNA in germinal epithelium | |
| IGFBP-1 | Constant | Decreased | Not detected | IHC weak |
| IGFBP-2 | Constant | Constant | In Leydig and Sertoli cells | IHC positive |
| IGFBP-3 | Increased in pT1, slightly decreased in pT2 and pT3 | Slightly increased in pT1, decreased in pT2 | mRNA in vessels | IHC weakly positive |
| IGFBP-4 | Slightly increased in pT1, decreased in pT2 | Decreased | mRNA in vessels, Leydig cells, and connective tissue | IHC weak |
| IGFBP-5 | Decreased in pT1 and pT2 | Decreased | mRNA in connective tissue and little in Leydig cells | IHC positive |
| IGFBP-6 | Constant | Decreased | mRNA peritubular and interstitial | IHC weak |
IHC = immunohistochemistry.