Figure 1.

Glioma cell migration is stimulated by Pyk2 expression and inhibited by FAK expression. SF767 (A and C) or G112 (B and D) glioma cell lines were infected with recombinant adenoviruses encoding Pyk2 or FAK at the indicated MOI and migration rate on laminin measured with a monolayer migration assay. Migration rates of infected cells were derived from slopes of lines from measurements of radii of cell population versus time. Cells were plated on 1 µg/ml laminin (A and B) or on 10 µg/ml laminin (C and D). Values represent mean ± SD of 10 replicate samples. Expression of Pyk2 or FAK was detected by immunoblotting of cell lysates with anti-FLAG or anti-HA antibodies, respectively. *P < .05.