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. 2005 May;7(5):537–543. doi: 10.1593/neo.04685

Figure 3.

Figure 3

Epo signaling mediates invasion in HNSCC cell lines. (a) 22B cells display higher invasive potential as assayed with Matrigel coated Boyden chambers for a 48-hour period under serum-free conditions (*P < .05). (b) Exogenous rhEpo promotes cell invasion of O22 and 22B cells through Matrigel-coated Boyden chambers under serum-free conditions (48 hours; *P < .05). (c) Exogenous rhEpo promotes cell invasion of hepatoma (Hep3B) and prostate (DU145) cancer cell lines through Matrigel-coated Boyden chambers under serum-free conditions (48 hours; *P < .05). (d) Exogenous rhEpo (10 U/ml) treatment enhances phosphorylation of JAK2 and this activation is blocked with AG490 (20 µM; *P < .05). (e) Epo (10 U/ml)-induced invasion in O22 cells is blocked with AG490 (20 µM) treatment (*P < .05). (f) Basal invasion of 22B cells is reduced with AG490 (20 µM) treatment only under serum-free conditions (*P < .05).