FIG. 4.

RT-PCR analysis of the gntK and gntH expression in a gntR gntH double disruptant and a gntR single disruptant. (A to C) YU577 (gntR::Tn10 gntH::cml) (A) and YU563 (gntR::cml gntH⁺) (B and C) were grown in LB medium at 37°C for 2 h and further incubated for 2 h in the presence of 0.5% gluconate (GA) or 5-ketogluconate (5KGA). Total RNAs were then prepared and subjected to RT-PCR analysis with primers specific for gntK (A and B) or gntH (C) as described in Materials and Methods. The numerals represent cycles of PCR. (D) rRNA was used as a control. Total RNAs (10 μg) in panels A through C were used.