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. 2003 Mar;185(6):1783–1795. doi: 10.1128/JB.185.6.1783-1795.2003

FIG. 5.

FIG. 5.

Repression of the gntK expression by GntH derived from a multicopy plasmid, pGNTH18. (A and B) YU563 (gntR::cml gntH+) harboring pACYC177 as a control plasmid or pGNTH18 bearing gntH was grown in LB medium at 37°C for 2 h and further incubated for 2 h in the presence of 0.5% gluconate (GA). Total RNAs were then prepared and subjected to RT-PCR analysis with primers specific for gntK as described in Materials and Methods. The numbers above the lanes represent cycles of PCR. (C) rRNA was used as a control. Lanes 1 and 2 contain total RNAs (10 μg) from cells harboring pACYC177 and cells harboring pGNTH18 grown in the medium without GA, respectively, and lanes 3 and 4 contain total RNAs from cells harboring pACYC177 and harboring pGNTH18 grown with GA, respectively.