FIG. 6.

Change of gntK expression along with the cell cycle in the medium containing gluconate. (A to D) NK7049 (A), YU632 (idnO::cml) (B), and YU564 (gntH::cml) (C) were grown in minimum medium containing gluconate as the sole carbon source at 37°C for 4 h (early logarithmic phase), 9 h (late logarithmic phase), and 14 h (early stationary phase). Total RNAs were then prepared and subjected to RT-PCR analysis with primers specific for gntK (A to C) or gntH (D) as described in Materials and Methods. The numbers above the lanes represent cycles of PCR. (E) rRNA was used as a control. Lanes 1 to 3 contain total RNAs (10 μg) from NK7049, YU632, and YU564, respectively.