TABLE 2.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Reference or source |
|---|---|---|
| Strains | ||
| TG1 | supE hsdΔ5 thi Δ(lac-proAB) F′ traΔ36 proAB lacIqlacZΔM15 | 29 |
| CT690 | recB21 recC22 thi-1 thr-1 leu-6 lacY1 mxl-1 xyl-1 ara14 galK2 his-4 proA2 argF3 rpsL31 tsx-33 sup-37 sbcB15 | M, Tsuda |
| P90C | ara Δ(lac-pro) thi | 19 |
| NK7049 | ΔlacX74 galOP308 rpsL | 33 |
| YU506 | NK7049 λ Φ(gntK-lacZ) | This work |
| YU507 | NK7049 λ Φ(gntT-lacZ) | This work |
| YU563 | NK7049 gntR::cml | This work |
| YU564 | NK7049 gntH::cml | This work |
| YU565 | YU563 λ Φ(gnK-lacZ) | This work |
| YU566 | YU564 λ Φ(gnK-lacZ) | This work |
| YU567 | YU563 λ Φ(gntT-lacZ) | This work |
| YU568 | YU564 λ Φ(gntT-lacZ) | This work |
| YU577 | NK7049 gntR::Tn10 gntH::cml | This work |
| YU578 | YU577 λ Φ(gntK-lacZ) | This work |
| YU579 | YU577 λ Φ(gntT-lacZ) | This work |
| YU632 | NK7049 idnO::cml | This work |
| Plasmids | ||
| pACYC177 | Ampr Kanr | 5 |
| pCB192 | AmprlacZ galK | 31 |
| pUC119 | AmprlacZα | 35 |
| pMBL18 | AmprlacZα | 22 |
| pRS551 | Ampr Kanr promoterless lacZ | 33 |
| pKF18k | Kanr (dual amber mutant) lacZα | 9 |
| pGEX4T-1 | Ampr GST gene | Amersham Pharmacia Biotech |
| pGNT2 | pACYC177 with gntV, idnD, idnO, gntW, and gntH | This work |
| pGNT5 | pBR322 with gntK | 12 |
| pGNTT20 | pBR322 with gntT | 13 |
| pGNTK-CAT1 | pKK232-8 with the BamHI-PstI fragment from pGNT5 | 12 |
| pYY2 | Frameshift mutation of the bla gene on pACYC177 | 37 |
| pGNTR1 | pACYC177 with gntR | 12 |
| pGNTH | pACYC177 with gntH | This work |
| pGNTR18 | pMBL18 with gntR | This work |
| pGNTH119 | pUC119 with the 1.7-kb DraI fragment from pGNT2 | This work |
| pGNTH18 | pMBL18 with gntH | This work |
| pGNTR177 | pACYC177 with gntR | This work |
| pGNTR177-CM | pGNTR177 with cml | This work |
| pGNTH-DIS2 | pUC119 with gntH | This work |
| pGNTH-DIS2-CM | pGNTH-DIS2 with cml | This work |
| pBRIDNO | pBR322 with idnO | This work |
| pBRIDNO-CM | pBRIDNO with cml | This work |
| pGST-GNTR | pGEX4T-1 with gntR | This work |
| pGST-GNTH | pGEX4T-1 with gntH | This work |
| pGNTT-LAC4 | pCB192 with the 460-bp Sau3AI fragment from pGNTT20 | 12 |
| pGNTK-LAC | pCB192 with the 1.2-kb SmaI-HindIII fragment from pGNTK-CAT1 | This work |
| pKFGNTK | pKF18k with the 1.2-kb SmaI-HindIII fragment from pGNTK-CAT1 | This work |
| pGNTK-LACMR1 | pGNTK-LAC derivative with mutations at the R1 sequence of the gntK operator (MR1) | This work |
| pGNTK-LACMR2 | pGNTK-LAC derivative with mutations at the R2 sequence of the gntK operator (MR2) | This work |
| pGNTK-LACMR12 | pGNTK-LAC derivative with mutations at the R1 and R2 sequences of the gntK operator (MR12) | This work |
| pRSGNTK | pRS551 with the 500-bp fragment including the promoter-operator of gntK | This work |
| pRSGNTT | pRS551 with the 460-bp fragment including the promoter-operator of gntT | This work |