TABLE 3.

Expression of single-copy gntK-lacZ and gntT-lacZ operon fusions on the genome in LB medium

Strain	β-Galactosidase activity (Miller units)a at:
	4 h			24 h
	No addition	GAb addition	5KGAb addition	No addition	GAb addition	5KGAb addition
gntK-lacZ
YU578 (gntR::Tn10 gntH::cml)
+ pACYC177c	6,200 ± 150	3,800 ± 160	4,700 ± 580	9,800 ± 800	5,500 ± 100	6,100 ± 100
+ pGNTR18c	86 ± 15	3,500 ± 140	1,600 ± 130	43 ± 3.0	1,800 ± 100	530 ± 10
+ pGNTH18c	99 ± 0	59 ± 3.5	63 ± 1.6	130 ± 10	34 ± 3.0	45 ± 4.0
YU506 (gntR+ gntH+)	42 ± 2.0	2,800 ± 700	420 ± 70	23 ± 2.0	1,600 ± 100	91 ± 29
YU565 (gntR::cml gntH+)	4,600 ± 1,000	3,200 ± 26	2,300 ± 300	12,000 ± 1,000	7,200 ± 300	3,300 ± 100
YU566 (gntR+ gntH::cml)	34 ± 1.0	2,300 ± 100	1,000 ± 100	44 ± 2.0	1,300 ± 100	430 ± 50
YU578 (gntR::Tn10 gntH::cml)	4,900 ± 800	3,500 ± 300	4,900 ± 1,100	6,500 ± 300	5,400 ± 600	7,700 ± 1,700
gntT-lacZ
YU579 (gntR::Tn10 gntH::cml)
+ pACYC177	1,400 ± 43	730 ± 25	1,100 ± 19	1,800 ± 100	1,100 ± 200	1,100 ± 100
+ pGNTR18	76 ± 11	830 ± 6.3	660 ± 61	45 ± 1.0	470 ± 0	280 ± 10
+ pGNTH18	170 ± 0	140 ± 5.0	130 ± 0	290 ± 30	100 ± 0	110 ± 10
YU507 (gntR+ gntH+)	38 ± 9.0	730 ± 100	230 ± 30	27 ± 1.0	220 ± 60	94 ± 2.0
YU567 (gntR::cml gntH+)	1,300 ± 100	600 ± 90	480 ± 50	2,400 ± 400	1,400 ± 100	810 ± 70
YU568 (gntR+ gntH::cml)	33 ± 2.0	560 ± 30	360 ± 30	26 ± 2.0	270 ± 30	230 ± 20
YU579 (gntR::Tn10 gntH::cml)	1,000 ± 70	510 ± 50	550 ± 50	1,800 ± 200	1,100 ± 200	1,100 ± 100

^aReported values are means and standard deviations of more than two independent experiments performed in triplicate.

^b0.5% gluconate (GA) or 5-ketogluconate (5KGA) was added after a 2-h incubation.

^cpACYC177, pGNTR18, and pGNTH18 were used as the control plasmid, the plasmid bearing gntR, and the plasmid bearing gntH, respectively.