FIG. 4.
Electrophoretic profile of PCR products with primers pairs P21 × P22 and P82 × P83 on chromosomal DNA from different strains. (A) Products of PCR with primer pair P21 × P22 on chromosomal DNA from strains NZ131 (lane 4), NZ131 Rec− (lane 5), M1 (strain 370) (lane 6), M3 (strain 364) (lane 7), and M5 (strain 455) (lane 8). Lanes 1 and 2 are controls without chromosomal DNA, and lane 3 is molecular size standard (1-kb standard; Promega). (B) Lane 1, molecular size standards; lane 2, P82 × P83 on NZ131; lane 3, P82 × P83 on NZ131 Rec−; lane 4, P82 × P83 on OK50; lane 5, control with no DNA. (C) Lane 1, P21 × P22 on NZ131; lane 2, P82 × P83 on NZ131; lane 3, molecular size control; lanes 4 and 5, controls with no DNA.