Table 2.

Oligonucleotide primers used for K5 and K14 chain mutants

Construct	Oligonucleotide primers	pET11a	pEGFPK14-C1
Keratin 14
1A Lys01Met	5′-CTG GTG GGC AGT GAG AtG GTG ACC ATG CAG AAC CTC-3′	X
	3′-GAC CAC CCG TCA CTC TaC CCA TGG TAC GTC TTG GAG-5′
1A Arg10Leu	5′-CAG AAC CTC AAT GAC CtC CTG GCC TCC TAC CTG GAC-3′
	3′-GTC TTG GAG TTA CTG GaG GAC CGG AGG ATG GAC CTG-5′	X
1A Lys17Met	5′-CCT CCT ACC TGG ACA tGG TGC GTG CTC TGG AGG AGG-3′	X
	3′-GGA GGA TGG ACC TGT aCC ACG CAC GAG ACC TCC TCC-5
1A Arg19Leu	5′-TAC CTG GAC AAG GTG CtT GCT CTG GAG GAG GCC-3′	X
	3′-ATG GAC CTG TTC CAC GaA CGA GAC CTC CTC CGG-5′
1A Glu22Asp	5′-GGT GCG TGC TCT GGA cGA GGC CAA CGC CGA CCT GG-3′	X	X
	3′-CCA GCG ACG AGA CCT gCT CCG GTT GCG GCT GGA CC-5′
1A Glu22Ala	5′-GGT GCG TGC TCT GGc AGA GGC CAA CGC CGA CCT GG-3′	X	X
	3′-CCA GCG ACG AGA CCg TCT CCG GTT GCG GCT GGA CC-5′
1B Arg12Met	5′-ACC ATT GAG GAC CTG AtG GAA CAA GAT TCT CAC AG-3′	X
	3′-TGG TAA CTC CTG GAC TaC CTT GTT CTA AGA GTG TC-5′
1B Asp56Glu	5′-CGC ATG AGT GTG GAA GCC GAa ATC AAT GGC CTG CGC AGG GT-3′		X
	3′-GCG TAC TCA CAC CTT CGG CTt TAG TTA CCG GAC GCG TCC CA-5′
1B Asp56Ala	5′-CGC ATG AGT GTG GAA GCC GcC ATC AAT GGC CTG CGC AGG GT-3′		X
	3′-GCG TAC TCA CAC CTT CGG CgG TAG TTA CCG GAC GCG TCC CA-5′
1B Glu75Ala	5′-TGG CCA GAG CTG ACC TGG cGA TGC AGA TTG AGA GCC TGA-3′	X
	3′-ACC GGT CTC GAC TGG ACC gCT ACG TCT AAC TCT CGG ACT-5′
1B Glu79Ala	5′-TGG AGA TGC AGA TTG cGA GCC TGA AGG AGG AGC TGG-3′
	3′-ACC TCT ACG TCT AAC gCT CGG ACT TCC TCC TCG ACC-5′	X
1B Lys82Glu	5′-ATG CAG ATT GAG AGC CTG gAG GAG GAG CTG GCC TAC CTG-3′	X
	3′-TAC GTC TAA CTC TCG GAC cTC CTC CTC GAC CGG ATG GAC-5
1B Glu84Asp	5′-GAG AGC CTG AAG GAG GAc CTG GCC TAC CTG AAG AAG AAC C-3′	X	X
	3′-CTC TCG GAC TTC CTC CTg GAC CGG ATG GAC TTC TTC TTG G-5′
1B Glu84Lys	5′-GAG AGC CTG AAG GAG aAA CTG GCC TAC CTG AAG AAG AAC C-3′		X
	3′-CTC TCG GAC TTC CTC tTT GAC CGG ATG GAC TTC TTC TTG G-5′
2B Lys04Met	5′-GAA TGG CTC TTC ACC AtG ACA GAG GAG CTG AAC CGC GA-3′	X
	3′-CTT ACC GAG AAG TGG TaC TGT CTC CTC GAC TTG GCG CT-5′
2B Lys23Ile	5′-CTG GTG CAG AGC GCC Att AGC GAG ATC TCG GAG CTC CGG-3′	X	X
	3′-GAC CAC GTC TCG CCG Taa TCG CTC TAG AGC CTC GAG GCC-5′
2B Glu39Asp	5′-CCA TGC AGA ACC TGG AGA TTG AcC TGC AGT CCC AGC TCA GCA-3′	X
	3′-GGT ACG TCT TGG ACC TCT AAC TgG ACG TCA GGG TCG AGT CGT-5′
2B Glu39Ala	5′-CCA TGC AGA ACC TGG AGA TTG cGC TGC AGT CCC AGC TCA GCA-3′	X	X
	3′-GGT ACG TCT TGG ACC TCT AAC gCG ACG TCA GGG TCG AGT CGT-5′
2B Glu39Lys	5′-CCA TGC AGA ACC TGG AGA TTa AGC TGC AGT CCC AGC TCA GCA-3′	X
	3′-GGT ACG TCT TGG ACC TCT AAt TCG ACG TCA GGG TCG AGT CGT-5′
2B Glu76Ala	5′-GAG ATG ATT GGC AGC GTG GcG GAG CAG CTG GCC CAG CTC C-3′	X
	3′-CTC TAC TAA CCG TCG CAC CgC CTC GTC GAC CGG GTC GAC G-5′
2B Lys100Met	5′-CAG GAG CTC ATG AAC ACC AtG CTG GCC CTG GAC GTG GAG-3′	X
	3′-GTC CTC GAG TAC TTG TGG TaC GAC CGG GAC CTG CAC CTC-5′
2B Glu104Ala	5′-GGA CGT GAA GAC GCG GCT GGc GCA GGA GAT CGC CAC CTA CCG-3′	X	X
	3′-CCT GCA CTT CTG CGC CGA CCg CGT CCT CTG GCG GTG GAC GGC-5′
2B Glu104Asp	5′-GGA CGT GAA GAC GCG GCT GGA cCA GGA GAT CGC CAC CTA CCG-3′	X
	5′-CCT GCA CTT CTG CGC CGA CCT gGT CCT CTG GCG GTG GAT GGC-5′
2B Glu106Asp	5′-CGC GGC TGG AGC AGG AcA TCG CCA CCT ACC GCC GCC-3′	X	X
	3′-GCG CCG ACC TCG TCC TgT AGC GGT GGA TGG CGG CGG-5′
2B Glu106Ala	5′-CGC GGC TGG AGC AGG cGA TCG CCA CCT AGG GCC GCC-3′		X
	3′-GCG CCG ACC TCG TCC gCT AGC GGT GGA TGG CGG CGG-5′
2B Glu106Lys	5′-CGC GGC TGG AGC AGa AGA TCG CCA CCT AGG GCC GCC-3′	X	X
	3′-GCG CCG ACC TCG TCt TCT AGC GGT GGA TGG CGG CGG-5′
2B Thr109Asp	5′-CTG GAG CAG GAG ATC GCC gaC TAC CGC CGC CTG CTG GAG GAG-3′	X
	3′-GAC CTC GTC CTC TAG CGG ctG ATG GCG GCG GAC GAC CTC CTC-5′
2B Thr109Phe	5′-CTG GAG CAG GAG ATC GCC ttC TAC CGC CGC CTG CTG GAG GAG-3′	X
	3′-GAC CTC GTC CTC TAG CGG aaG ATG GCG GCG GAC GAC CTC CTC-5′
2B Thr109Lys	5′-CTG GAG CAG GAG ATC GCC aaa TAC CGC CGC CTG CTG GAG GAG-3′	X
	3′-GAC CTC GTC CTC TAG CGG ttt ATG GCG CGC CTG GAC CTC CTC-5′
2B Arg111Leu	5′-CAG GAG ATC GCC ACC TAC CtC CGC CTG CTG GAG GGC CGA-3′	X	X
	3′-GTC CTC TAG CGG TGG ATG GaG GCG GAC GAC CTC CCG GCT-5′
Keratin 5
1A Lys17Met	5′-TGG AGC AGC AGA ACA tGG TTC TGG ACA CCA AGT GGA CC-3′	X
	3′-ACC TCG TCG TCT TGT aCC AAG ACC TGT GGT TCA CCT GG-5′
1A Glu22Asp	5′-GGT GCG GTT CCT GGA cCA GCA GAA CAA GGT TCT GG-3′	X
	3′-CCA CGC CAA GGA CCT gGT CGT CTT GTT CCA AGA CC-5′
1A Glu22Ala	5′-GGT GCG GTT CCT GGc GCA GCA GAA CAA GGT TCT GG-3′	X
	3′-CCA CGC CAA GGA CCg CGT CGT CTT GTT CCA AGA CC-5′
1B Glu54Ala	5′-AAG CGT ACC ACT GCT GcG AAT GAG TTT GTG ATG CTG-3′	X
	3′-TTC GCA TGG TGA CGA CgC TTA CTC AAA CAC TAC GAC-5′
1B Lys71Ile	5′-GCT GCC TAC ATG AAC AtA GTG GAG CTG GAG GCC AA-3′	X
	3′-CGA CGG ATG TAC TTG TaT CAC CTC GAC CTC CGG TT-5′
1B Asp79Ala	5′-CTG GAG GCC AAG GTT GcT GCA CTG ATG GAT GAG ATT-3′	X
	3′-GAC CTC CGG TTC CAA CgA CGT GAC TAC CTA CTC TAA-5′
1B Glu84Asp	5′-GCA CTG ATG GAT GAc ATT AAC TTC ATG AAG ATG TTC-3′	X
	3′-CGT GAC TAC CTA CTg TAA TTG AAG TAC TTC TAC AAG-5′
1B Glu84Ala	5′-GCA CTG ATG GAT GcG ATT AAC TTC ATG AAG ATG TTC-3′	X
	3′-CGT GAC TAC CTA CgC TAA TTG AAG TAC TTC TAC AAG-5′
1B Lys89Met	5′-GAG ATT AAC TTC ATG AtG ATG TTC CTT GAT GCG GAG CTG-3′	X
	3′-CTC TAA TTG AAG TAC TaC TAC AAG GAA CTA CGC CTC GAC-5′
2B Asp18Glu	5′-GAG ATT AAC TTC ATG AAG AaG TTC CTT GAT GCG GAG CTG-3′	X
	3′-CTC TAA TTG AAG TAC TTC TtC AAG GAA CTA CGC CTC GAC-5′
2B Asp18Ala	5′-CTG GCC GGC ATG GCG ATG cCC TCC GCA ACA CCA AGC-3′	X
	3′-GAC CGG CCG TAC CGC TAC gGG AGG CGT TGT GGT TCG-5′
2B Arg20Leu	5′-GGC ATG GCG ATG ACC TCC tCA ACA CCA AGC ATG AGA-3′	X
	3′-CCG TAC CGC TAC TGG AGG aGT TGT GGT TCG TAC TCT-5′
2B Lys23Ile	5′-GAT GAC CTC CGC AAC ACC Atc CAT GAG ATC ACA GAG ATG-3′	X
	3′-CTA CTG GAG GCG TTG TGG Tag GTA CTC TAC TGT CTC TAC-5′
2B Glu25Lys	5′-GAC CTC CGC AAC ACC AAG CAT aAG ATC ACA GAG ATG AAC-3′	X
	3′-CTG GAG GCG TTG TGG TTC GTA tTC TAG TGT CTC TAC TTG-5′
2B Glu25Ala	5′-GAC CTC CGC AAC ACC AAG CAT GcG ATC ACA GAG ATG AAC-3′	X
	3′-CTG GAG GCG TTG TGG TTC GTA CgC TAG TGT CTC TAC TTG-5′
2B Lys44Glu	5′-GCC GAG ATT GAC AAT GTC gAG AAA CAG TGC GCC AAT CTG-3′	X
	3′-CGG CTC TAA CTG TTA CAG cTC TTT GTC ACG CGG TTA GAC-5′
2B Asp104Glu	5′-CAC CCA GCT GGC CCT GGA aGT GGA GAT CGC CAC TTA CCG-3′	X
	3′-GTG GGT CGA CCG GGA CCT tCA CCT CTA GCG GTG AAT GGC-5′
2B Asp104Ala	5′-CAC CCA GCT GGC CCT GGc AGT GGA GAT CGC CAC TTA CCG-3′	X
	3′-GTG GGT CGA CCG GGA CCg TCA CCT CTA GCG GTG AAT GGC-5′
2B Asp104Lys	5′-CAC CCA GCT GGC CCT Gaa AGT GGA GAT CGC CAC TTA CCG-3′	X
	3′-GTG GGT CGA CCG GGA Ctt TCA CCT CTA GCG GTG AAT GGC-5′
2B Glu106Asp	5′-GGC CCT GGA CGT GGA cAT CGC CAC TTA CCG CAA GCT-3′	X
	3′-CCG GGA CCT GCA CCT gTA GCG GTG AAT GGC GTT CGA-5′
2B Glu106Ala	5′-GGC CCT GGA CGT GGc GAT CGC CAC TTA CCG CAA GCT-3′	X
	3′-CCG GGA CCT GCA CCg CTA GCG GTG AAT GGC GTT CGA-5′
2B Arg111Asp	5′-ATC GCC ACT TAC gaC AAG CTG CTG GAG GGC GAG-3′	X
	3′-TAG CGG TGA ATG ctG TTC GAC GAC CTC CCG CTC-5′
2B Arg111Leu	5′-ATC GCC ACT TAC CtC AAG CTG CTG GAG GGC GAG-3′	X
	3′-TAG CGG TGA ATG GaG TTC GAC GAC CTC CCG CTC-5′