Table 5.

Summary of molecular stabilities of double-mutant chain mixtures in concentrated urea solutions

	Composition		Urea concentration (M) of half disassembly
	K5	K14	Tetramer	Dimer
1A	Lys17Met	Lys17Met	5.5	7.5
	Lys17Met	Arg19Leu	4	5
	Glu22Ala	Arg19Leu	4.5	5.5
	Glu22Ala	Glu22Ala	4	6
1B	Glu54Ala	Asp56Ala	5	8
	Glu54Ala	Glu75Ala	4.5	7
	Glu54Ala	Glu79Ala	4.5	6
	Glu54Ala	Lys82Glu	5	7
	Lys71Ile	Glu75Ala	4.5	7
	Lys71Ile	Lys82Glu	4.5	6.5
	Lys71Ile	Glu84Lys	4	6
	Asp79Ala	Glu75Ala	4	6
	Asp79Ala	Lys82Glu	<1	<1
	Asp79Ala	Glu84Lys	<1	<1
	Glu84Ala	Asp56Ala	4.5	6
	Glu84Ala	Glu75Ala	1	2
	Glu84Ala	Glu79Ala	<1	<1
	Glu84Ala	Lys82Glu	<1	<1
	Lys89Met	Asp56Ala	4	6.5
	Lys89Met	Glu75Ala	<1	<1
	Lys89Met	Glu79Ala	<1	<1
	Lys89Met	Lys82Glu	<1	<1
2B	Asp18Ala	Glu39Ala	6	8.5
	Asp18Ala	Glu76Ala	5	8
	Asp18Ala	Glu106Asp	2	3
	Glu25Ala	Glu39Ala	5.5	8
	Glu25Ala	Glu76Ala	5.5	8
	Glu25Ala	Glu106Asp	2	3
	Glu25Ala	Thr109Lys	1	3
	Arg111Asp	Glu106Asp	2	4
	Arg111Asp	Thr109Lys	<1	<1