FIG. 3.

Thymine starvation in an E. coli thyA mutant induces mazEF-mediated cell death. Viability (% survivors) is plotted against times of growth of E. coli strains KL742thyA748::Tn10 (WT), KL742thyA748::Tn10ΔmazEF (ΔmazEF), and KL742thyA748::Tn10ΔclpP (clpP −) at 37°C in M9 liquid medium lacking thymine. Cell survival was determined as described in the legend to Fig. 1a.