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. 2003 Mar;185(6):1923–1934. doi: 10.1128/JB.185.6.1923-1934.2003

FIG. 1.

FIG. 1.

Construction of plasmids that express asRNA directed toward the downregulation of CoAT. (A) Construction of pCTFA2AS, pCTFB1AS, and pCOAT11AS. For each plasmid, the locations and directions of transcription of the relevant genes are indicated (arrows). Relevant restriction sites are shown. Abbreviations: thl promoter, promoter region for the thiolase gene of C. acetobutylicum strain ATCC 824; ctfA, CoAT subunit A gene; ctfB, CoAT subunit B gene; adc, AADC gene; MLSr, macrolide-lincosamide-streptogramin B resistance gene; repL, pIM13 origin of replication; AMPr, ampicillin resistance gene; ColE1, ColE1 origin of replication. All genes and plasmids are not drawn to scale. (B) Genetic organization of the sol operon. Abbreviations: aad, alcohol/aldehyde dehydrogenase structural gene; sol operon, polycistronic message that includes the mRNA for aad, ctfA, and ctfB. Lines with arrows represent the location and direction of each transcript.