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. 2003 Mar;185(6):1923–1934. doi: 10.1128/JB.185.6.1923-1934.2003

FIG. 6.

FIG. 6.

Downregulation of CtfA and CtfB subunits of CoAT by CoAT-asRNA. (A) CoAT Western blots from the transitional, early stationary, and stationary phases of C. acetobutylicum static-flask fermentations. The CtfA and CtfB subunits of CoAT as well as the closest marker bands are indicated for all blots. The culture phase from which the samples on each blot were taken are indicated below each blot. Lanes 1, 5, and 10, biotinylated protein marker from the horseradish peroxidase protein marker detection pack from New England Biolabs; lanes 2, 6, and 11, ATCC 824(pSOS95del); lanes 3, 8, and 12, ATCC 824(pCTFB1AS); lane 7, degenerate ATCC 824(pSOS95del); lanes 4, 9, and 13, ATCC 824(pCOAT11AS). (B) Percent downregulation of CtfA and CtfB subunits of CoAT by CoAT-asRNA. The percent downregulation of CtfA and CtfB in ATCC 824(pCTFB1AS) (▪) and 824(pCOAT11AS) (□) was calculated as the percent decrease of the desired gel band intensity of Western blots in the asRNA-expressing strain compared to that in the plasmid control strain [824(pSOS95del)] at the same culture phase. The standard error of measurement was calculated from two to four different blots.