TABLE 2.
Oligonucleotides used for PCR
| Primer | Oligonucleotide sequencea (5′-3′) | Use |
|---|---|---|
| ADCUP | GTAAATATAAATAAATAGGACTAGAGGCGA | Upstream primer for adc38, adc68, and adc100 |
| ADC38DOWN | CTGTCCGCTTTCTGGATCCCAA | Downstream primer for adc38 |
| ADC68DOWN | GCTTCATTAGCATCTAAGGCT | Downstream primer for adc68 |
| ADC100DOWN | CACAGTTTAAAAAACCTCCAATCA | Downstream primer for adc100 |
| CTFAUP | ATCTTAAATACTCGAGTTAAGAATTT | Upstream primer for ctfa1 and ctfa2 |
| CTFADOWN1 | GATCCGCCTGCTCGAGTTGGATCCACTAGAG | Downstream primer for ctfa1 |
| CTFADOWN2 | ATAGAAGGTGGATCCGGCCTCGAGTACAAT | Downstream primer for ctfa2 |
| CTFBUP | AGCAATGACCCTCGAGTTCTTAT | Upstream primer for ctfb1 |
| CTFBDOWN1 | CGAAAAATGTGCCGTCTCGAGGATCCGTTGT | Downstream primer for ctfb1 |
a
Boldface letters in the oligonucleotide sequences indicate nucleotide substitutions used to generate useful restriction sites near the termini of the PCR products. Relevant restriction sites are underlined.