TABLE 3.

Fermentation characteristics^a of strains for the asRNA downregulation of AADC in pH-controlled bioreactor experiments^b

Strain	No. of expts	Growth rate (h−1)	Maximum A600	Acetone concn	Acetone concn/maximum A600c	Butanol concn	Butanol concn/maximum A600c	Peak acetate concn/maximum A600c	Final acetate concn/maximum A600c	Peak butyrate concn/maximum A600c	Final butyrate concn/maximum A600c	Butanol/acetate ratio
ATCC 824	2	0.47 ± 0.07	7.63 ± 0.33	79 ± 4	10.4 ± 0.0	139 ± 9	18.2 ± 0.0	9.8 ± 0.0	9.1 ± 0.2	11.3 ± 0.4	6.4 ± 0.6	1.75 ± 0.03
ATCC 824(SOS95del)	6	0.45 ± 0.03	5.56 ± 0.42	49 ± 2	9.5 ± 1.0	93 ± 3	17.3 ± 1.6	15.9 ± 1.8	14.7 ± 1.2	13.4 ± 1.1	10.3 ± 0.7	1.91 ± 0.08
ATCC 824(pADC38AS)	2	0.43 ± 0.04	6.34 ± 0.04	50 ± 1	7.9 ± 0.2	118 ± 2	18.6 ± 1.8	17.2 ± 1.2	16.3 ± 0.9	11.9 ± 0.2	5.5 ± 1.8	2.34 ± 0.17
ATCC 824(pADC68AS)	2	0.42 ± 0.02	5.56 ± 0.19	47 ± 7	8.5 ± 0.9	101 ± 10	18.0 ± 1.1	17.4 ± 0.3	17.0 ± 0.5	14.2 ± 0.2	9.2 ± 0.7	2.14 ± 0.09
ATCC 824(pADC100AS)	2	0.44 ± 0.06	6.43 ± 0.42	73 ± 1	11.5 ± 0.9	141 ± 1	22.0 ± 1.6	15.9 ± 0.4	14.5 ± 0.4	12.5 ± 0.5	7.0 ± 0.2	1.92 ± 0.01

^aMean ± standard error of the mean.

^bConcentrations are millimolar.

^cRatios of product concentrations over the maximum cell density (A₆₀₀) were calculated in order to normalize some key data for comparison purposes.