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Regulation of the mgtA::lacZ transcriptional fusion by the PhoQ-T48X mutants. (A) E. coli MG1607 cells carrying the pPROQHis-T48X plasmids were grown in LB broth or LB broth supplemented with 10 mM MgCl2. β-Galactosidase activities are expressed in Miller milliunits. Data are the means of triplicate values with standard deviations. WT, wild type; Ctl., control. (B) Western blot analysis of the expression level of the various PhoQ-T48X mutants.
