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. 2005 Jun;7(6):563–574. doi: 10.1593/neo.04607

Figure 3.

Figure 3

Cas IIgly induced the loss of mitochondrial membrane potential and the release of apoptogenic factors. (A) Mitochondrial membrane potential from control and 24-hour Cas IIgly-treated glioma C6 cells was measured by accumulated R123 fluorescence determined by flow cytometry. The data represent the mean ± SD (*P ≤ .05, **P ≤ .001, and ***P ≤ .0001) of three independent determinations. (B) Western blot analysis for cyt c, the cytosolic fraction obtained from control and 24-hour Cas IIgly-treated C6 cells (left panel). The bar graphs indicate the relative amounts of cyt c normalized to the respective actin level (right panel). Each bar represents the mean of three independent experiments. (C) Caspase-3 activity is presented as fold increases over control. Each bar represents a mean of three independent experiments. (D) Western blot analysis for AIF and Endo G in the nuclear fraction obtained from control and 24 h Cas IIgly-treated C6 cells (left panel). The bar graphs indicate the relative amounts of AIF and Endo G normalized to the respective laminin B level (right panel). Each bar represents the mean of three independent experiments.