Skip to main content
. 2005 Jun;7(6):563–574. doi: 10.1593/neo.04607

Figure 5.

Figure 5

Increase in cellular ROS is associated with apoptosis induced by Cas IIgly. (A) Effects of NAC on DNA of 24-hour Cas IIgly-treated cells in the presence of 20 mM NAC. DNA extraction was performed as described in Materials and Methods section. The marker is a 1-kb extension ladder. (B) Immunocytochemistry of AIF in control C6 cells, treated with Cas IIgly and Cas IIgly +20 mM NAC for 24 hours (left panel). The figures shown are representative of at least three different experiments for each experimental condition. Original magnification, x20. The bar graphs indicate the mean of three independent determinations (left panel). Quantitative estimation of AIF-positive nuclei at different doses was determined by counting five fields of x20 for each determination. (C) Caspase-3 activity in control C6 cells, treated with Cas IIgly and Cas IIgly +20 mM NAC for 24 hours. Caspase-3 activity was determined as described in Materials and Methods section. Caspase-3 activities were presented as fold increases over control. Each bar represents the mean ± SD (**P ≤ .001 and ***P ≤ .0001) of three independent experiments.