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. 2000 Oct;11(10):3559–3572. doi: 10.1091/mbc.11.10.3559

Figure 5.

Figure 5

Analysis of E3-13.7 distribution by ultrathin cryosectioning and immunoelectron microscopy. (A–D) Infected ERwt cells were incubated with HRP (1 mg/ml) for 30 min before fixation and cryopreservation. Sections were costained for E3-13.7 (10-nm gold particles) and HRP (5-nm gold particles). (E–J) Infected ERwt cells were costained for E3-13.7 (10-nm gold particles) and EGF receptor (5-nm gold particles). Large arrowheads, colocalized 5-nm and 10-nm gold particles; small arrowheads, E3-13.7 localized to peripheral endosomes. In F–J, arrows indicate lack of E3-13.7 colocalization with EGF receptor. e, endosome; mvb, multivesicular body; pm, plasma membrane. Bars, 0.1 μm.