Figure 6.

Cleavage of caspase 9 is regulated by the activation of PAK1. MCF10A cells were transfected with wild-type, active, or dominant-negative PAK1 constructs. Cells were grown for 48 hours posttransfection, counted, and split into two plates: one regular culture dish and one polyhema-coated Petri dish for a further 24 hours. Cell lysates were then processed for cleavage of caspase 9, as revealed by appearance of a 37-kDa fragment (A). Blots were also probed with 9e10 to detect myc-tagged PAK1 constructs (B).