TABLE 1.
Strains of E. coli and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| Strain | ||
| AB2847 | aroB thi malT tsx | 21 |
| DH5α | F−hsdR17(rK−mK+) supE44 thi-1 gyrA relA1 recA1 endA1 Δ(argF lacZYA) U169 (φ80 ΔlacZ M15) λ− | Stratagene |
| AA93 | F−araD139 ΔlacU169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR thi aroB Δfec | 43 |
| WA1031 | AB2847 fepA fecA | 61 |
| CO1031 | WA1031 tonB | 22 |
| H2300 | AB2847 Δ(tonB trp) | K. Hankte |
| BL21 | F−hsd gal | 54 |
| HE2350 | GM1 tolQ exb::Tn10 [exbD] tonB | 5 |
| IS1031 | WA1031 lac::Tn10 | 22 |
| AS418 | AB2847 tonB fecB::lacZ | A. Sauter |
| Plasmids | ||
| pT7-7 | ori ColE1, phage T7 gene promoter; Ampr | 55 |
| pIS711 | pT7-7 fecA | 28 |
| pMON33 | pT7-7 fecA(D80C) | This study |
| pMON2 | pT7-7 fecA(A81C) | This study |
| pMON34 | pT7-7 fecA(L82C) | This study |
| pMON4 | pT7-7 fecA(T83C) | This study |
| pMON5 | pT7-7 fecA(V84C) | This study |
| pMON44 | pT7-7 fecAΔTonbox | This study |
| pMON26 | pT7-7 fecAFhubox | This study |
| pMON32 | pT7-7 fecAFepbox | This study |
| pMON37 | pT7-7 fecBCDE | This study |
| pMON28 | pT7-7 fecA fecBCDE | This study |
| pMON55 | pT7-7 fecAΔTonbox fecBCDE | This study |
| pHG575/6 | pSC101 derivative, Cmr | 56 |
| pMMO203 | pHSG576 fecIR | 53 |
| pLCIRA | pHSG576 fecIR fecA | This study |
| pMON52 | pHSG576 fecIR fecA(D80C) | This study |
| pMON7 | pHSG576 fecIR fecA(A81C) | This study |
| pMON8 | pHSG576 fecIR fecA(L82C) | This study |
| pMON9 | pHSG576 fecIR fecA(T83C) | This study |
| pMON10 | pHSG576 fecIR fecA(V84C) | This study |
| pMON45 | pHSG575 fecIR fecAΔTonbox | This study |
| pMON27 | pHSG575 fecI fecA with FhuA TonB box (DTITV) | This study |
| pMON29 | pHSG575 fecIR fecA with FepA TonB box (DTITVV) | This study |
| pBAD18 | ori pBR322 araC pBAD promoter; Ampr | 19 |
| pMON47 | pBAD18 fecA(D80C) | This study |
| pMON48 | pBAD18 fecA(A81C) | This study |
| pMON49 | pBAD18 fecA(L82C) | This study |
| pMON50 | pBAD18fecA(T83C) | This study |
| pMON51 | pBAD18fecA(V84C) | This study |
| pSU19 | ori P15A Cmr | 36 |
| pTonBC18A | pSU19 tonBC18A exbB exbD | 11 |
| pTonBQ160C | pSU19 tonBQ160C exbB exbD | 11 |
| pTonBQ162C | pSU19 tonBQ162C exbB exbD | 11 |
| pTonBY163C | pSU19 tonBY163C exbB exbD | 11 |
| pMON22 | pHSG575 tonBC18A | This study |
| pMON23 | pHSG575 tonBQ160C | This study |
| pMON24 | pHSG575 tonBQ162C | This study |
| pMON25 | pHSG575 tonBY163C | This study |
| pMON56 | pSU19 tonBQ160C exbB exbD(D25N) | This study |
| pMON57 | pSU19 tonBQ162C exbB exbD(D25N) | This study |
| pMON58 | pSU19 tonBY163C exbB exbD(D25N) | This study |
| pTonBC18ANP | pTonBC18A; without tonB native promoter | This study |
| pCH10 | pWSK29 exbB exbD(D25N) | 8 |
| pGFPA′ | GFP fused to fecA promoter | S. Enz |