TABLE 5.
Abilities of TonB and TonB/Cys to support FecA-mediated growth promotion where ferric citrate is the sole iron source
| Straina | Plasmid | TonB sequence | Growth zone (mm)b
|
|||
|---|---|---|---|---|---|---|
| 1 mM | 10 mM | 100 mM | 1 M | |||
| AB2847 | 13 | 20 | 34 | 45 | ||
| WA1031 | 0 | 0 | (15) | (23) | ||
| H2300 | 0 | 0 | (12) | (25) | ||
| pHSG575 | 0 | 0 | (12) | (23) | ||
| pMON22 | C18A | 14 | 24 | 35 | 50 | |
| pMON23 | Q160C | 15 | 20 | 35 | 52 | |
| pMON24 | Y162C | 15 | 26 | 39 | 53 | |
| pMON25 | Y163C | 0 | 0 | 14 | 25 | |
a
Growth measurements of plasmids were determined in strain H2300 (AB2847 ΔtonB).
b
Growth was measured as the diameters of the growth zones around 6-mm-diameter filter disks that had been saturated with 10 μl of the indicated concentrations of sodium citrate. Numbers in parentheses indicate growth zones with low cell densities.