Figure 4.

FR-Preconditioned Blocking of Greening and Germination Responses in the rep1-1 Mutant.

(A) FR-preconditioned blocking of greening. The seedlings were grown on Murashige and Skoog (1962) medium for 5 days in FR light (7 μmol m⁻² sec⁻¹) and then irradiated with white light (WL; 15 μmol m⁻² sec⁻¹) for the times indicated. Each measurement was from 50 seedlings. The error bars indicate standard deviations of three independent measurements.

(B) PhyA-dependent germination responses. Germination frequencies of wild type (WT), phyA-211, and rep1-1 mutant seeds were measured. The seeds were treated with FR light for 15 min just after imbibition and returned to darkness for 2 days. Seeds were then incubated in darkness for an additional 5 days, without (Dark) or with treatment with FR light (7 μmol m⁻² sec⁻¹) for 15 min. Each measurement was performed with at least 150 seeds. The error bars indicate the standard deviations of three independent measurements.