Figure 6.

Molecular Cloning of the REP1 Gene.

(A) Genomic structure of the REP1 gene. The locations of start codon (ATG) and stop codon (TGA) are shown. The triangles indicate the positions of T-DNA insertions in the rep1-1 and rep1-2 mutants, respectively. The structure of cDNA is shown along with the regions for the bHLH motif. Filled boxes indicate exons, and lines between boxes indicate introns.

(B) Deduced amino acid sequence of the REP1 gene (GenBank accession number AF288287). The bHLH motif is boxed, and the putative bipartite nuclear localization signal is underlined.

(C) RNA gel blot analysis of rep1 mutants. Total RNA was extracted from seedlings of the wild type and rep1-1 and rep1-2 mutants that had been grown in darkness for 4 days and then subjected to RNA gel blot analysis with a REP1 cDNA probe. Ten micrograms of total RNA was loaded onto each lane. The 18S rRNA probe was used as a loading control.