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Subcellular Localization of the REP1–GUS Protein.
A construct encoding REP1 cDNA fused to GUS driven by the cauliflower mosaic virus 35S promoter was introduced into onion epidermal cells by particle bombardment.
(A) After 24 hr of incubation under W light, the cells were stained for GUS activity. The arrow indicates the nucleus.
(B) Fluorescence staining with 4′,6-diamidino-2-phenylindole identifies the nucleus (arrow).
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