Figure 8.

Expression Analysis of the REP1 Gene.

(A) RNA gel blot analysis of CAB and REP1 transcripts in the wild type (WT) and rep1-1 mutants. The seedlings were grown on Murashige and Skoog (1962) medium for 4 days in darkness and then irradiated with FR light (7 μmol m⁻² sec⁻¹) for the indicated times before harvest. The 18S rRNA was used as a loading control.

(B) Quantitative measurement of the relative amounts of CAB and REP1 transcripts shown in (A). The values denotes relative expressions and were calculated by first normalizing each signal against that for 18S rRNA and then against the amount of expression in the wild type for each particular gene. The signals were quantified with a PhosphoImager (Fuji, FLA 2000).