Effects of eds5 on PR-1, PR-2, PR-5, and PDF1.2 Gene Expression in the cpr Mutants.
PR-1, PR-2, PR-5, and PDF1.2 gene-specific probes were used for RNA gel blot analysis of the indicated genotypes. The UBQ5 transcript was used as a loading standard. RNA was extracted from 3-week-old soil-grown plants. RNA gel blot analysis was performed at both Duke and Massachusetts General Hospital with similar results. c1e5, cpr1 eds5; c5e5, cpr5 eds5; c6e5, cpr6 eds5; WT, wild-type BGL2-GUS transgenic line.