Figure 1.

Constructs for Transient Expression.

secGFP is derived from the ER-targeted GFP-HDEL by replacing the C-terminal tetrapeptide with a c-Myc epitope tag. N-secGFP, N-GFP- HDEL, and N-ST-GFP are derived from secGFP, GFP-HDEL, and ST-GFP (Boevink et al., 1998), respectively, by addition of an N-terminal peptide containing an N-glycosylation site. The steady state location and the fluorescence characteristics of each GFP in this location are indicated at right. All constructs were inserted under control of an enhanced CaMV promoter in an Agrobacterium binary vector (Hawes et al., 2000). NGT, single-letter amino acid code; ST, sialyltransferase.