Figure 5.

sec-GFP Accumulation Phenotype of AtRab1b(N121I) Is Rescued by Coexpression of Wild-Type AtRab1b but Not AtRab8c.

(A) Low-magnification images of epidermal cells expressing secGFP, either alone or in combination with the AtRab1b derivative, as indicated at bottom left. secGFP accumulates in the presence of AtRab1b(N121I) (bottom left), but when wild-type and mutant AtRab1b are coexpressed (bottom right), secGFP accumulation is suppressed to amounts similar to those observed in controls that express only secGFP (top left) or secGFP and wild-type AtRab1b alone (top right).

(B) Low-magnification (at top) and high-magnification (at bottom) confocal images of epidermal cells expressing GFP-HDEL alone or in combination with AtRab1b and AtRab1b(N121I), as specified at bottom left of each image. Coexpression with the AtRab1b constructs affected neither the intensity nor the pattern of GFP-HDEL accumulation, as indicated by the upper and lower images, respectively.

(C) Low-magnification images of epidermal cells expressing secGFP. The wild-type AtRab8c does not induce intracellular accumulation of secGFP (left) and cannot suppress the effects of AtRab1b(N121I) (right).

for all images; for the upper images and 10 μm for the lower images; for both images.