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Fos-luciferase reporter assay. (A) Luciferase activity corresponds to transforming activity in cells transfected with Neu TM-shift mutants. The fold induction relative to mock transfected cells is set at 1. The positive control NeuNT exhibited a 5.4-fold induction. (B) Neu immunoblot of cell lysates demonstrates equivalent expression of the receptor in each sample. The arrow indicates the mobility of receptor monomers.
