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Restoration of activity to the inactive −2 ΔEC-shift mutant by insertion of additional Val residues in the add-back series. (A) The sequence of each transmembrane domain in this series of mutants is presented. (B) Transforming activity presented as the number of foci/10-cm plate/2.5 μg of DNA. Six plates from three independent experiments were counted. The SE of the mean is shown. The mock and positive NeuNT controls are described in MATERIALS AND METHODS.
