Figure 6.

(A) Transformation assay of PDGFR-β constructs containing the TM-shift domains. Transforming activity is presented as the number of foci/10-cm plate/1.25 μg of DNA. Four plates were counted for each mutant and the SE of the mean is shown. The negative and positive controls, PDGFR-β/neu and PDGFR-β/neu*, respectively, are described in MATERIALS AND METHODS. (B) Partial summary of RTK families is presented, indicating the transmembrane (TM) and juxtamembrane domains (JM). Separation between the transmembrane domain and the kinase domain varies from ∼35 to 75 residues, depending upon the specific RTK.