Table 2.
Tissue Tropism of Hybrid Geminiviruses with Mismatched versus Matched BL1 and BR1 Movement Proteins
| Genotypeb
|
Cells Infectedc
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Characteristicsa | AL23 | BRi | BL1 | BR1 | V | M | T | M/T % | χ2 |
| BGMV-based hybrid viruses | |||||||||
| Matched MPs (wild-type BGMV) | B | B | B | B | 314 | 3 | 317 | 0.95 | a |
| Mismatched MPs | B | T | B | T | 230 | 22 | 252 | 8.73 | b |
| Mismatched MPs | T | T | B | T | 260 | 11 | 271 | 4.06 | c |
| Matched BGMV MPs | T | T | B | B | 383 | 387 | 770 | 50.26 | d |
| Matched TGMV MPs | T | T | T | T | 293 | 307 | 600 | 51.17 | d |
| TGMV-based hybrid viruses | |||||||||
| Matched BGMV MPs | B | B | B | B | 233 | 2 | 235 | 0.85 | a |
| Mismatched MPs | B | T | B | T | 226 | 1 | 227 | 0.44 | a |
| Mismatched MPs | T | T | B | T | 211 | 22 | 233 | 9.44 | b |
| Matched BGMV MPs | T | T | B | B | 403 | 294 | 697 | 42.18 | e |
| Matched MPs (wild-type TGMV) | T | T | T | T | 279 | 644 | 923 | 69.77 | f |
Matched movement proteins (MPs) are defined as combinations of BL1 and BR1 from the same virus; mismatched combinations have BGMV BL1 paired with TGMV BR1. Controls were wild-type viruses.
Hybrid viruses contained the AL23 ORFs, BRi noncoding region, and BL1 and BR1 ORFs from either BGMV (B) or TGMV (T), as indicated.
Infected cells that could be unambiguously identified as vascular associated (V) or mesophyll (M) were scored. The total number (T) of infected cells (where T = V + M) and the percentage of the total that were mesophyll cells (M/T %) are also given. Mesophyll invasion efficiencies that were not significantly different (P > 0.05 by χ2 test) are indicated by like letters under χ2.