Table 1.
Seed Viability and Endosperm Development after Crosses between fie-1/FIE Heterozygous Seed Parents and Wild-Type Pollen Parents Compared with Crosses between Wild-Type Plants
| Progeny Characteristics | fie-1/FIEa | FIE/FIEa | FIE/FIEb |
|---|---|---|---|
| Expected frequency of embryo genotypes (%) | 50 | 50 | 100 |
| Observed seed phenotypes | 45% shrivelled (n = 219) | 55% plump (n = 219) | 98% plump (n = 126) |
| Seed weight (μg) | No data | 26.0 (n = 120) | 20.2 (n = 60) |
| Germination (%) | 0 (n = 99) | 92 (n = 120) | 100 (n = 20) |
| Maximum no. of PE nuclei (±se) | 408 ± 33 (n = 3) | 447 ± 33 (n = 3) | 429 ± 31 (n = 3) |
| Endosperm cellularization | None at 10 DAP (n = 10) | 5–6 DAP (n = 5) | 5–6 DAP (n = 10) |
| Chalazal endospermc | 10–15X (n = 10) | 1X (n = 5) | 1X |
a
Cross: fie-1 × FIE/FIE.
b
Cross: FIE/FIE × FIE/FIE.
c
Area of maximum cross-section relative to the wild type (1×).