Table 3.
Seed Viability and Endosperm Development after Crosses between fie-1/FIE Heterozygous Seed Parents and fie-1/FIE Heterozygous Pollen Parents Hemizygous for the METI a/s Construct
| Progeny Characteristics (with respect to FIE) | FIE/FIEa FIE/fie-1a | fie-1/FIEa | fie-1/fie-1a |
|---|---|---|---|
| Expected frequency of embryo genotypes (%) | 25 25 | 25 | 25 |
| Observed seed phenotypes at 10 DAP (%)b | 49 Maternal excess (n = 123) | 25 Paternal excess (n = 123) | 26 Lethal paternal excess (n = 123) |
| Seed weight (μg) | Small seeds 17.9 (n = 96) | Large seeds 32.9 (n = 125) | Shrivelled seeds 10.6 (n = 59) |
| Latest embryo stage observed | Mature | Mature | Heart–torpedo |
| Chalazal endosperm at 12 DAP | None visible | None visible | Persistent and filling seed |
a
Cross: fie-1/FIE × fie-1/FIE; METI a/s.
b
Segregation of maturing and aborted seeds consistent with a 3:1 ratio, χ2 = 0.068, P > 0.1.