Table 4.
Autonomous Endosperm Development in Unpollinated fie-1/FIE Heterozygotes with Normal and Reduced DNA Methylation
| Embryo Sac Genotypes (with Respect to FIE) |
fie-1a | FIEa | fie-1b | FIEb | |
|---|---|---|---|---|---|
| Expected frequency of embryo sac genotypes |
50% | 50% | 50% | 50% | |
| Observed phenotypes | 47% Autonomous endosperm (n = 186) |
53% No development (n = 186) |
51% Autonomous endosperm (n = 233) 60% Type 1c 40% Type 2d (n = 30) (n = 30) |
49% No development (n = 233) |
|
| Maximum no. of PE nuclei (±se) |
199 ± 44 (n = 4) |
—e | 532 ± 117 (n = 3) |
697 ± 42 (n = 3) |
— |
| Endosperm cellularization | None at 10 DAE f (n = 20) | — | 5–6 DAE (n = 25) |
7–8 DAE (n = 20) |
— |
| Chalazal endospermg | 0 (n = 20) | — | 0.01–0.05X (n = 5) |
5–10X (n = 5) |
— |
a
Parent (emasculated): fie-1/FIE.
b
Parent (emasculated): fie-1/FIE; MET1 a/s.
c
Cellularized peripheral endosperm; chalazal endosperm absent (see text for details).
d
Cellularized peripheral endosperm; large chalazal endosperm present (see text for details).
e
Dash indicates no development.
f
DAE, days after emasculation.
g
Area of maximum cross-section relative to the wild type (1×).